Review



stat3  (Cell Signaling Technology Inc)


Bioz Verified Symbol Cell Signaling Technology Inc is a verified supplier
Bioz Manufacturer Symbol Cell Signaling Technology Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 97
      Buy from Supplier

    Structured Review

    Cell Signaling Technology Inc stat3
    Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, <t>P-Stat3,</t> Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.
    Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 2535 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat3/product/Cell Signaling Technology Inc
    Average 97 stars, based on 2535 article reviews
    stat3 - by Bioz Stars, 2026-03
    97/100 stars

    Images

    1) Product Images from "Huangqi-Danshen Decoction Against Renal Fibrosis in UUO Mice via TGF-β1 Induced Downstream Signaling Pathway"

    Article Title: Huangqi-Danshen Decoction Against Renal Fibrosis in UUO Mice via TGF-β1 Induced Downstream Signaling Pathway

    Journal: Drug Design, Development and Therapy

    doi: 10.2147/dddt.s457100

    Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, P-Stat3, Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.
    Figure Legend Snippet: Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, P-Stat3, Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Techniques Used: Expressing, Western Blot

    Figure 8 Effects of HDD on key targets expression in TGF-β1-induced HK-2 cells. (A) Western blot images of α-SMA, P-EGFR, EGFR, PI3K, P-Stat3, and Stat3 expression in HK-2 cells stimulated with TGF-β1 or/and HDD. (B) Quantitative analysis of α-SMA, EGFR (phosphorylated/total), PI3K, and Stat3 (phosphorylated/total) expression, normalized to α-Tubulin. (C) Western blot images of α-SMA, P-EGFR, and EGFR expression in TGF-β1-induced HK-2 cells treated with NSC228155 or/and HDD. (D) Quantitative analysis of α-SMA and EGFR (phosphorylated/total) expression, normalized to α-Tubulin. (E) Western blot images of α-SMA, P-Stat3, and Stat3 expression in TGF-β1-induced HK-2 cells treated with colivelin or/and HDD. (F) Quantitative analysis of α-SMA and Stat3 (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 3–5 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI3K, phosphoinositide 3-kinase; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.
    Figure Legend Snippet: Figure 8 Effects of HDD on key targets expression in TGF-β1-induced HK-2 cells. (A) Western blot images of α-SMA, P-EGFR, EGFR, PI3K, P-Stat3, and Stat3 expression in HK-2 cells stimulated with TGF-β1 or/and HDD. (B) Quantitative analysis of α-SMA, EGFR (phosphorylated/total), PI3K, and Stat3 (phosphorylated/total) expression, normalized to α-Tubulin. (C) Western blot images of α-SMA, P-EGFR, and EGFR expression in TGF-β1-induced HK-2 cells treated with NSC228155 or/and HDD. (D) Quantitative analysis of α-SMA and EGFR (phosphorylated/total) expression, normalized to α-Tubulin. (E) Western blot images of α-SMA, P-Stat3, and Stat3 expression in TGF-β1-induced HK-2 cells treated with colivelin or/and HDD. (F) Quantitative analysis of α-SMA and Stat3 (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 3–5 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI3K, phosphoinositide 3-kinase; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Techniques Used: Expressing, Western Blot

    Figure 9 Schematic diagram showing potential mechanisms by which HDD antagonizes renal fibrosis. Abbreviations: Akt, polyserine/threonine kinase; ECM, extracellular matrix; EGFR, epidermal growth factor receptor; EMT, epithelial-mesenchymal transition; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; SIRT1, silent information regulator 1; Stat3, signal transduction and transcriptional activator 3; TGF-β1, transforming growth factor-β1; TIMP2, tissue inhibitors of metalloproteinase 2.
    Figure Legend Snippet: Figure 9 Schematic diagram showing potential mechanisms by which HDD antagonizes renal fibrosis. Abbreviations: Akt, polyserine/threonine kinase; ECM, extracellular matrix; EGFR, epidermal growth factor receptor; EMT, epithelial-mesenchymal transition; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; SIRT1, silent information regulator 1; Stat3, signal transduction and transcriptional activator 3; TGF-β1, transforming growth factor-β1; TIMP2, tissue inhibitors of metalloproteinase 2.

    Techniques Used: Transduction



    Similar Products

    stat3  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc stat3
    Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, <t>P-Stat3,</t> Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.
    Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat3/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    stat3 - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    mouse anti stat3  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc mouse anti stat3
    Primary and secondary antibodies used in the study
    Mouse Anti Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti stat3/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    mouse anti stat3 - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    rabbit anti stat3  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc rabbit anti stat3
    Antibodies used in the study
    Rabbit Anti Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti stat3/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    rabbit anti stat3 - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    stat3 families  (Cell Signaling Technology Inc)
    95
    Cell Signaling Technology Inc stat3 families
    ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and <t>JAK2/STAT3</t> signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments
    Stat3 Families, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat3 families/product/Cell Signaling Technology Inc
    Average 95 stars, based on 1 article reviews
    stat3 families - by Bioz Stars, 2026-03
    95/100 stars
    		  Buy from Supplier
    transam® stat3 & stat family kits  (Active Motif)
    90
    Active Motif transam® stat3 & stat family kits
    ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and <t>JAK2/STAT3</t> signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments
    Transam® Stat3 & Stat Family Kits, supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transam® stat3 & stat family kits/product/Active Motif
    Average 90 stars, based on 1 article reviews
    transam® stat3 & stat family kits - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier
    stat3 mouse cell signaling  (Cell Signaling Technology Inc)
    97
    Cell Signaling Technology Inc stat3 mouse cell signaling
    ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and <t>JAK2/STAT3</t> signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments
    Stat3 Mouse Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/stat3 mouse cell signaling/product/Cell Signaling Technology Inc
    Average 97 stars, based on 1 article reviews
    stat3 mouse cell signaling - by Bioz Stars, 2026-03
    97/100 stars
    		  Buy from Supplier
    elisa-based transcription factor assay kit (stat3 family)  (Active Motif)
    90
    Active Motif elisa-based transcription factor assay kit (stat3 family)
    ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and <t>JAK2/STAT3</t> signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments
    Elisa Based Transcription Factor Assay Kit (Stat3 Family), supplied by Active Motif, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa-based transcription factor assay kit (stat3 family)/product/Active Motif
    Average 90 stars, based on 1 article reviews
    elisa-based transcription factor assay kit (stat3 family) - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, P-Stat3, Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Journal: Drug Design, Development and Therapy

    Article Title: Huangqi-Danshen Decoction Against Renal Fibrosis in UUO Mice via TGF-β1 Induced Downstream Signaling Pathway

    doi: 10.2147/dddt.s457100

    Figure Lengend Snippet: Figure 7 Effects of HDD on key targets expression in the kidneys of UUO mice. (A) Western blot images of P-EGFR, EGFR, PI3K, P-Stat3, Stat3, MMP9, MMP2, P-Src, and Src expression in kidney tissue. (B) Quantitative analysis of EGFR (phosphorylated/total), PI3K, Stat3 (phosphorylated/total), MMP9, MMP2, and Src (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 6 mice per group, ns = no significance, *P < 0.05, **P < 0.01, ***P < 0.001. (C) Representative IHC images of PI3K, P-Stat3, MMP9, and P-Src. All images are shown at identical magnification, × 400, scale bar = 100 μm. (D) Quantitative analysis of PI3K, P-Stat3, MMP9, and P-Src expression in IHC. Data are expressed as the mean ± SEM, n = 5 mice per group, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; Src, non-receptor tyrosine kinase Src; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Article Snippet: The membranes were reacted with primary antibodies against fibronectin (FN, ab2413, Abcam), α-smooth muscle actin (α-SMA, A5228, Sigma-Aldrich), transforming growth factor-β1 (TGF-β1, ab179695, Abcam), Phospho-Smad2/3 (P-Smad2/3, #8828, CST), Smad2/3 (#8685, CST), phosphoinositide 3-kinase (PI3K, #4249, CST), Phospho- signal transducer and activator of transcription 3 (P-Stat3, #9145, CST), Stat3 (#9139, CST), Phospho-Src (P-Src, #2101, CST), Src (#2109, CST), Phospho-epidermal growth factor receptor (P-EGFR, #3777S, CST), EGFR (#4267S, CST), matrix metalloproteinase-9 (MMP9, 10375- 2-AP, Proteintech), matrix metalloproteinase-2 (MMP2, 10373-2-AP, Proteintech), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004-1-1g, Proteintech) at 4 °C overnight.

    Techniques: Expressing, Western Blot

    Figure 8 Effects of HDD on key targets expression in TGF-β1-induced HK-2 cells. (A) Western blot images of α-SMA, P-EGFR, EGFR, PI3K, P-Stat3, and Stat3 expression in HK-2 cells stimulated with TGF-β1 or/and HDD. (B) Quantitative analysis of α-SMA, EGFR (phosphorylated/total), PI3K, and Stat3 (phosphorylated/total) expression, normalized to α-Tubulin. (C) Western blot images of α-SMA, P-EGFR, and EGFR expression in TGF-β1-induced HK-2 cells treated with NSC228155 or/and HDD. (D) Quantitative analysis of α-SMA and EGFR (phosphorylated/total) expression, normalized to α-Tubulin. (E) Western blot images of α-SMA, P-Stat3, and Stat3 expression in TGF-β1-induced HK-2 cells treated with colivelin or/and HDD. (F) Quantitative analysis of α-SMA and Stat3 (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 3–5 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI3K, phosphoinositide 3-kinase; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Journal: Drug Design, Development and Therapy

    Article Title: Huangqi-Danshen Decoction Against Renal Fibrosis in UUO Mice via TGF-β1 Induced Downstream Signaling Pathway

    doi: 10.2147/dddt.s457100

    Figure Lengend Snippet: Figure 8 Effects of HDD on key targets expression in TGF-β1-induced HK-2 cells. (A) Western blot images of α-SMA, P-EGFR, EGFR, PI3K, P-Stat3, and Stat3 expression in HK-2 cells stimulated with TGF-β1 or/and HDD. (B) Quantitative analysis of α-SMA, EGFR (phosphorylated/total), PI3K, and Stat3 (phosphorylated/total) expression, normalized to α-Tubulin. (C) Western blot images of α-SMA, P-EGFR, and EGFR expression in TGF-β1-induced HK-2 cells treated with NSC228155 or/and HDD. (D) Quantitative analysis of α-SMA and EGFR (phosphorylated/total) expression, normalized to α-Tubulin. (E) Western blot images of α-SMA, P-Stat3, and Stat3 expression in TGF-β1-induced HK-2 cells treated with colivelin or/and HDD. (F) Quantitative analysis of α-SMA and Stat3 (phosphorylated/total) expression, normalized to GAPDH. Data are expressed as the mean ± SEM, n = 3–5 independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001. Abbreviations: α-SMA, α-smooth muscle antibody; EGFR, epidermal growth factor receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; PI3K, phosphoinositide 3-kinase; Stat3, signal transducer and activator of transcription 3; TGF-β1, transforming growth factor-β1.

    Article Snippet: The membranes were reacted with primary antibodies against fibronectin (FN, ab2413, Abcam), α-smooth muscle actin (α-SMA, A5228, Sigma-Aldrich), transforming growth factor-β1 (TGF-β1, ab179695, Abcam), Phospho-Smad2/3 (P-Smad2/3, #8828, CST), Smad2/3 (#8685, CST), phosphoinositide 3-kinase (PI3K, #4249, CST), Phospho- signal transducer and activator of transcription 3 (P-Stat3, #9145, CST), Stat3 (#9139, CST), Phospho-Src (P-Src, #2101, CST), Src (#2109, CST), Phospho-epidermal growth factor receptor (P-EGFR, #3777S, CST), EGFR (#4267S, CST), matrix metalloproteinase-9 (MMP9, 10375- 2-AP, Proteintech), matrix metalloproteinase-2 (MMP2, 10373-2-AP, Proteintech), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004-1-1g, Proteintech) at 4 °C overnight.

    Techniques: Expressing, Western Blot

    Figure 9 Schematic diagram showing potential mechanisms by which HDD antagonizes renal fibrosis. Abbreviations: Akt, polyserine/threonine kinase; ECM, extracellular matrix; EGFR, epidermal growth factor receptor; EMT, epithelial-mesenchymal transition; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; SIRT1, silent information regulator 1; Stat3, signal transduction and transcriptional activator 3; TGF-β1, transforming growth factor-β1; TIMP2, tissue inhibitors of metalloproteinase 2.

    Journal: Drug Design, Development and Therapy

    Article Title: Huangqi-Danshen Decoction Against Renal Fibrosis in UUO Mice via TGF-β1 Induced Downstream Signaling Pathway

    doi: 10.2147/dddt.s457100

    Figure Lengend Snippet: Figure 9 Schematic diagram showing potential mechanisms by which HDD antagonizes renal fibrosis. Abbreviations: Akt, polyserine/threonine kinase; ECM, extracellular matrix; EGFR, epidermal growth factor receptor; EMT, epithelial-mesenchymal transition; MMP2, matrix metalloproteinase-2; MMP9, matrix metalloproteinase-9; PI3K, phosphoinositide 3-kinase; SIRT1, silent information regulator 1; Stat3, signal transduction and transcriptional activator 3; TGF-β1, transforming growth factor-β1; TIMP2, tissue inhibitors of metalloproteinase 2.

    Article Snippet: The membranes were reacted with primary antibodies against fibronectin (FN, ab2413, Abcam), α-smooth muscle actin (α-SMA, A5228, Sigma-Aldrich), transforming growth factor-β1 (TGF-β1, ab179695, Abcam), Phospho-Smad2/3 (P-Smad2/3, #8828, CST), Smad2/3 (#8685, CST), phosphoinositide 3-kinase (PI3K, #4249, CST), Phospho- signal transducer and activator of transcription 3 (P-Stat3, #9145, CST), Stat3 (#9139, CST), Phospho-Src (P-Src, #2101, CST), Src (#2109, CST), Phospho-epidermal growth factor receptor (P-EGFR, #3777S, CST), EGFR (#4267S, CST), matrix metalloproteinase-9 (MMP9, 10375- 2-AP, Proteintech), matrix metalloproteinase-2 (MMP2, 10373-2-AP, Proteintech), and glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 60004-1-1g, Proteintech) at 4 °C overnight.

    Techniques: Transduction

    Primary and secondary antibodies used in the study

    Journal: Translational Lung Cancer Research

    Article Title: SRC and PIM1 as potential co-targets to overcome resistance in MET deregulated non-small cell lung cancer

    doi: 10.21037/tlcr-20-681

    Figure Lengend Snippet: Primary and secondary antibodies used in the study

    Article Snippet: Primary antibodies and secondary antibodies used are in . table ft1 table-wrap mode="anchored" t5 caption a7 Western blotting primary antibody Dilution Company and catalog number Mouse anti-beta-actin 1:5,000 Sigma Aldrich (#A5441) Rabbit anti-AKT 1:1,000 Cell signaling (#9272) Rabbit anti-Phospho-AKT (S473) 1:1,000 Cell signaling (#9271) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Rabbit anti-CDCP1 1:1,000 Cell signaling (#4115) Rabbit anti-Phospho-CDCP1 (Y707) 1:1,000 Cell signaling (#13111) Rabbit anti-EGFR 1:1,000 Cell signaling (#4267) Rabbit anti-Phospho-EGFR (Y1068) 1:1,000 Cell signaling (#3777) Rabbit anti-Phospho-EGFR (Y845) 1:1,000 Cell signaling (#6963) Rabbit anti-ERK1/2 1:1,000 Cell signaling (#9102) Rabbit anti-PhosphoERK1/2 (T202/Y204) 1:1,000 Cell signaling (#9101) Rabbit anti-cMET 1:1,000 Cell signaling (#8198) Rabbit anti-Phospho-cMET (Y1234-1235) 1:1,000 Cell signaling (#3077) Rabbit anti-Src 1:1,000 Cell signaling (#2109) Rabbit anti-Phospho-Src Family (Y416) 1:1,000 Cell signaling (#6943) Mouse anti-STAT3 1:1,000 Cell signaling (#9139) Rabbit anti-Phospho STAT3 (Y705) 1:1,000 Cell signaling (#9145) Mouse anti-YAP1 1:1,000 Cell signaling (#12395) Rabbit anti-Phospho YAP1 (Y357) 1:1,000 Abcam (#ab62751) Rabbit anti-AXL 1:1,000 Cell signaling (#8661) Donkey anti-Rabbit IgG HRP-linked 1:5,000 GE Healthcare (#NA934) Sheep anti-Mouse IgG HRP-linked 1:5,000 GE Healthcare (#NXA931) Open in a separate window caption a8 Primary and secondary antibodies used in the study Cell viability assay The EBC-1 human squamous lung cancer cell line with MET amplification, the Hs746T human gastric cancer cell line with MET exon 14 skipping mutation, and the H1993 human lung adenocarcinoma cell line harboring MET amplification were purchased from American Type Culture Collection (ATCC).

    Techniques:

    Antibodies used in the study

    Journal: Translational Lung Cancer Research

    Article Title: Targeting PKCι-PAK1 in EGFR-mutation positive non-small cell lung cancer

    doi: 10.21037/tlcr.2019.08.25

    Figure Lengend Snippet: Antibodies used in the study

    Article Snippet: Primary and secondary antibodies used in the study was shown in . table ft1 table-wrap mode="anchored" t5 caption a7 Western blotting primary antibody Dilution Company and Catalog number Mouse anti-Beta-actin 1:5,000 Sigma Aldrich (#A5441) Rabbit anti-AKT 1:1,000 Cell Signaling (#9272) Rabbit anti-Phospho-AKT (Ser473) 1:1,000 Cell Signaling (#9271) Rabbit anti-AXL 1:1,000 Cell Signaling (#8661) Rabbit anti-Phospho-AXL (Tyr702) 1:1,000 Cell Signaling (#5724) Rabbit anti-CDCP1 1:1,000 Cell Signaling (#4115) Rabbit anti-Phospho-CDCP1 (Tyr707) 1:1,000 Cell Signaling (#13111) Rabbit anti-EGFR 1:1,000 Cell Signaling (#4267) Rabbit anti-Phospho-EGFR (TYr1068) 1:1,000 Cell Signaling (#3777) Rabbit anti-ERK1/2 1:1,000 Cell Signaling (#9102) Rabbit anti-PhosphoERK1/2 (Thr202/Tyr204) 1:1,000 Cell Signaling (#9101) Rabbit anti-cMET 1:1,000 Cell Signaling (#8198) Rabbit anti-PAK1 1:1,000 Cell Signaling (#2602) Rabbit anti-Phospho-PAK1/2 (Ser144/Ser141) 1:1,000 Cell Signaling (#2606) Rabbit anti-PKCι 1:500 Abcam (#5282) Rabbit anti-Phospho-PKC iota/lamda (Thr555/Thr563) 1:1,000 Abcam (#5813) Rabbit anti-Src 1:1,000 Cell Signaling (#2109) Rabbit anti-Phospho-Src Family (Tyr416) 1:1,000 Cell Signaling (#6943) Rabbit anti-STAT3 1:1,000 Cell Signaling (#9363) Rabbit anti-Phospho-STAT3 (TYr705) 1:1,000 Cell Signaling (#9145) Mouse anti-YAP1 1:1,000 Cell Signaling (#12395) Donkey anti-Rabbit Ig HRP-linked 1:5,000 GE Healthcare (#NA934) Sheep anti-Mouse IgG HRP-linked 1:5,000 GE Healthcare (#NXA931) Open in a separate window caption a8 Antibodies used in the study

    Techniques:

    ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and JAK2/STAT3 signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

    doi: 10.1111/jcmm.14290

    Figure Lengend Snippet: ZNF143 knockdown in colon cancer cells primarily affects extracellular signal‐regulated kinase (ERK)/ and JAK2/STAT3 signalling pathways. (A) Cells were harvested and analysed by immunoblotting to examine signalling pathway components. (B, C) Cells were harvested and subcellular fractionation (B) and immunostaining (C) were performed to determine STAT3 activation. Images were taken with the Zeiss 780 confocal microscope. Results shown are representative of at least three independent experiments

    Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

    Techniques: Knockdown, Western Blot, Fractionation, Immunostaining, Activation Assay, Microscopy

    Stattic, a STAT3 inhibitor, attenuates IL‐8 expression in colon cancer cells in a ZNF143‐dependent manner. (A) Cells were treated with 5 μmol/L Stattic for the indicated time periods and harvested to determine STAT3 phosphorylation by immunoblotting. (B, C) Cells were treated with 0, 1, 2.5, 5, and 10 μmol/L Stattic for 6 hours (B) or 24 hours (C) and harvested to determine STAT3 phosphorylation (B) or IL‐8 expression (C) by immunoblotting. Results shown are representative of at least three independent experiments

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

    doi: 10.1111/jcmm.14290

    Figure Lengend Snippet: Stattic, a STAT3 inhibitor, attenuates IL‐8 expression in colon cancer cells in a ZNF143‐dependent manner. (A) Cells were treated with 5 μmol/L Stattic for the indicated time periods and harvested to determine STAT3 phosphorylation by immunoblotting. (B, C) Cells were treated with 0, 1, 2.5, 5, and 10 μmol/L Stattic for 6 hours (B) or 24 hours (C) and harvested to determine STAT3 phosphorylation (B) or IL‐8 expression (C) by immunoblotting. Results shown are representative of at least three independent experiments

    Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

    Techniques: Expressing, Phospho-proteomics, Western Blot

    Increased IL‐8 contributes to STAT3 activation and IL‐8 expression through its receptor, CXCR2, in colon cancer cells. (A, B) Cells were harvested and analysed for mRNA expression of IL‐8 by reverse transcription (RT)‐polymerase chain reaction (PCR) (A) or real‐time PCR (B). (C) Cells were harvested, and the expression of signalling pathway components was determined by immunoblotting. Cells were treated with SB225002, an inhibitor of CXCR1, Stattic, or DMSO (vehicle) for 6 hours prior to harvesting. Results shown are representative of at least three independent experiments. Data are expressed as means ± S.E. of at least three independent experiments. Statistical significance was assessed using unpaired Student's t tests (*** P < 0.0001 and * P < 0.002)

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Loss of zinc‐finger protein 143 contributes to tumour progression by interleukin‐8‐CXCR axis in colon cancer

    doi: 10.1111/jcmm.14290

    Figure Lengend Snippet: Increased IL‐8 contributes to STAT3 activation and IL‐8 expression through its receptor, CXCR2, in colon cancer cells. (A, B) Cells were harvested and analysed for mRNA expression of IL‐8 by reverse transcription (RT)‐polymerase chain reaction (PCR) (A) or real‐time PCR (B). (C) Cells were harvested, and the expression of signalling pathway components was determined by immunoblotting. Cells were treated with SB225002, an inhibitor of CXCR1, Stattic, or DMSO (vehicle) for 6 hours prior to harvesting. Results shown are representative of at least three independent experiments. Data are expressed as means ± S.E. of at least three independent experiments. Statistical significance was assessed using unpaired Student's t tests (*** P < 0.0001 and * P < 0.002)

    Article Snippet: Rabbit monoclonal antibodies against p ‐p38/p‐38, p ‐ERK/ERK, p ‐JNK/JNK, p‐ p65/p65‐NFκB and STAT3 families were obtained from Cell Signaling Technology, Inc (Beverly, MA).

    Techniques: Activation Assay, Expressing, Reverse Transcription, Polymerase Chain Reaction, Real-time Polymerase Chain Reaction, Western Blot